Acid red 52
Kiton Red S
CAS: 3520-42-1
Molecular Formula: C27H29N2NaO7S2
Acid red 52 - Names and Identifiers
| Name | Kiton Red S |
| Synonyms | C.I. 45100 XYLENE RED Acid red 52 Kiton Red S XYLENE RED B Acid Red 52 SULFORHODAMINE B C.I. Acid Red 52 sulforhodamine B KitonRedSC.I.14895 PHLOXINE RHODAMINE sulforhodamine b sodium salt SULFORHODAMINE B MONOSODIUM SALT sulforhodamine B monosodium salt sulforhodamine B cell culture tested Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt, sodium salt sodium 4-[6-(diethylamino)-3-(diethyliminio)-3H-xanthen-9-yl]benzene-1,3-disulfonate |
| CAS | 3520-42-1 |
| EINECS | 222-529-8 |
| InChI | InChI=1/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1 |
Acid red 52 - Physico-chemical Properties
| Molecular Formula | C27H29N2NaO7S2 |
| Molar Mass | 580.65 |
| Density | 1.472[at 20℃] |
| Melting Point | Dye content, 75% |
| Water Solubility | 95.3g/L at 20℃ |
| Solubility | methanol: 1mg/mL |
| Appearance | Powder |
| Color | Brown to Very Dark Purple |
| BRN | 3886008 |
| Storage Condition | room temp |
| MDL | MFCD00010180 |
| Physical and Chemical Properties | Dark red powder. Soluble in water, aqueous solution with yellow fluorescent blue red, adding sodium hydroxide solution for blue red. Soluble in ethanol. In concentrated sulfuric acid was orange yellow, diluted red. |
| Use | Applied to fluorescence staining technology. |
Acid red 52 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. |
| WGK Germany | 2 |
| RTECS | BP6750000 |
| FLUKA BRAND F CODES | 8 |
| TSCA | Yes |
| HS Code | 32129000 |
Acid red 52 - Reference
| Reference Show more | 1. Yu Jie, Yang Zhenguo, Chen Meizhen, etc. Apoptosis of HeLa cells induced by bioactive peptides from oyster and its mechanism [J]. Food Science, 2012(21):290-294. 2. [IF = 6.576] Huang Yan et al."Hsian-Tsao the Thermal Tolerance of Drosophila melangaster." Frontal in Nutrition. 2022 May;0:885 |
Acid red 52 - Introduction
Soluble in water, soluble in ethanol. The fluorescence is orange. Stable in nature and can be stored for a long time. The maximum absorption wavelength is 570nm and the maximum emission wavelength is 600nm.
Last Update：2022-10-16 17:24:49
Acid red 52 - Reference Information
| color index | 45100 |
| LogP | -2.2 at 20℃ |
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| introduction | acid red 52 is also called sulfonyl rhodamine B. Sulfonyl rhodamine B is an organic substance, dark red powder, soluble in water, and can be used for cell viability detection. Sulfonyl rhodamine B(sulforhodamine B,SRB) colorimetry using acid red 52 is a cell viability detection method established by SKEHAN in 1990. It has been widely used because of its advantages of rapidity, stable signal and low cost. Especially in the field of tumor research, the National Cancer Institute of the United States has listed it as one of the standard anti-tumor screening methods. |
| Application | Sulfonyl rhodamine B is a pink protein binding dye, which can bind to basic amino acids in cell proteins under weakly acidic conditions, and under weakly alkaline conditions, Sulfonyl rhodamine B can be dissolved, and the absorbance value reflects the protein content, representing the number of cells. |
| detection principle | sulfonyl rhodamine (Sulforhodamine B,SRB) is a water-soluble protein dye that can bind to basic amino acids in cells to form complexes. The amount of total protein in cells can reflect the number of cells. The absorbance values measured at a wavelength of 515nm showed a good linear relationship with the number of cells. |
| use | acid rose red B is used for dyeing wool, silk and nylon and direct printing of wool and silk fabrics. It is bright red with fluorescence, very delicate and charming, with good levelness, but poor sun fastness and sensitive to water stains. It is only suitable for woolen fabrics with low color fastness requirements. It can be combined with acid light yellow 2G to dye bright red wool products, and is also used for dyeing leather and paper. It is mainly used for dyeing silk, nylon, wool and other fabrics. It can dye monochrome or color matching It is applied to fluorescence dyeing technology. |
| production method | using m-hydroxydiethylaniline (m-diethylamino-phenol) and 2,4-disulfonic benzaldehyde (4-formyl-m-benzenedisulfonic acid) as raw materials, after condensation, Oxidation, salt product. The finished product is filtered, dried and crushed.. |
Last Update：2024-04-09 21:11:58
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